As a first test of the role of Wnt signaling in zebrafish sex determination, Sreenivasan et al. Zebrafish monosex population reveals female dominance in sex determination and earliest events of gonad differentiation. Wild sex in zebrafish: loss of the natural sex determinant in domesticated strains.
Polygenic sex determination system in zebra fish heart in Or Liew et al. Moreover, PCR-based screens and aCGH performed by a custom-designed tiling array were both unable to find general differences between the genome of the two sexes in two different zebrafish strains.
On the pie charts, checkered background indicates males, and solid background indicates females. Godwin J. We found that zebrafish reared at high density had approximately twenty per cent more males on average than those raised at middle or low densities indicating a modest effect of large differences in rearing density on sex determination in zebrafish Fig.
Experimental evidence for the cumulative effect of the allelic combinations was observed on sex-linked loci reported by Bradley and colleagues see Supplementary Table 4 of [ 24 ]. Ellegren H.
Pigmentation pathway evolution after whole-genome duplication in fish. A greater magnitude of septin signaling transcripts sept3 and sept8b encoding sperm tail proteins was identified in the testis. Thus, while previous studies showed the importance of germ cells for female development, this study identified that meiotic oocytes are the key germ cell-stage that promotes ovarian, and thus female, development in domesticated zebrafish.
By contrast, for animals that were determined as males, their early-stage oocytes undergo apoptosis as the gonads transition from a proto-ovary into a functional testis. Temperature-dependent sex determination in fish revisited: Prevalence, a single sex ratio response pattern, and possible effects of climate change.
In general, Wt1 encodes a zinc finger transcription factor, which is necessary for the development of different tissues including kidney, gonad, spleen and heart in fish [ 30 ]. View Article Google Scholar 4. The latter data prove that the FluoMEP assay is suitable for isolating sex-linked markers from genomes known to contain heteromorphic sex chromosomes.
Zebrafish sex is determined genetically Since molecular and breeding studies failed to identify heteromorphic sex chromosomes or their effect, we next sought to find out if genetic factors are involved in zebrafish sex determination.